7 ways to optimize your pyrosequences (PyroMark, Qiagen)

When talking or reading about pyrosequencing you're probably thinking immediately of the high-throughput 454 sequencing. This sequencing technique is based on the "sequencing by synthesis" principle and differs radically from the standard Sanger sequencing. It can be viewed as the qPCR version of standard PCR. This technique was commercialized by Pyrosequencing AB and has been further developed for high throughput sequencing by 454 Life Sciences. Qiagen acquired the pyrosequencing business line in 2008 and started developing the pyrosequence technique for low/medium throughput applications.  So, even though it is based on the same technology (see video below), the pyrosequencing I am talking about here is NOT the 454 system but the Qiagen system!

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