Publications

Publications in peer-reviewed journals

2022

Wang Y., Rensink A.H., Fricke U., Riddle M.C., Trent C., Van de Zande L., Verhulst E.C. (2022) Sexually dimorphic traits and male-specific differentiation are actively regulated by Doublesex during specific developmental windows in Nasonia vitripennis. bioRxiv (DOI: 10.1101/2020.04.19.048553) (accepted in Insect Biochemistry and Molecular Biology)
Beekman, M.M.*, Donner, S.H.*, Litjens, J.J.H., Dicke, M., Zwaan, B.J., Verhulst, E.C., Pannebakker, B.A. (2022) Aphids out of control: the role of heritable elements in the ineffective parasitoid biocontrol of aphids in Dutch greenhouse sweet pepper crops (accepted in Evolutionary Applications)
Wang Y., Sun W., Fleischmann S., Millar J.G., Ruther J., Verhulst, E.C. (2022) Silencing Doublesex
expression triggers three-level pheromonal feminization in Nasonia males. bioRxiv (DOI: https://doi.org/10.1101/2021.05.21.445141) (accepted in Proceedings of the Royal Society B: Biological Sciences)
Williams, A.T., Verhulst, E.C., Haverkamp, A. (2022) A unique sense of smell: development and evolution of a sexually-dimorphic antennal lobe. (accepted in Entomologia Experimentalis et Applicata)

2021

Xiong, X., Kelkar, Y.D., Geden, G.J., Zhang, G.J., Wang, Y., Jongepier, E., Martinson, E.O., Verhulst, E.C., Gadau, J., Werren, J.H. and Wang, X. (2021) Long-read assembly and annotation of the parasitoid wasp Muscidifurax raptorellus, a biological control agent for filth flies. (accepted in Frontiers in Genetics)
Visser S., Voleníková A., Nguyen P., Verhulst E.C., Marec F. (2021) A conserved role of the duplicated Masculinizer gene in sex determination of the Mediterranean flour moth, Ephestia kuehniella. PLoS Genet 17(8): e1009420. (DOI: https://doi.org/10.1371/journal.pgen.1009420)
Rougeot, J., Wang, Y., Verhulst, E.C. (2021) Effect of using Green Fluorescent Protein double-stranded RNA as non-target negative control in Nasonia vitripennis RNA interference assays. Experimental Results, Volume 2 , e11 (DOI: https://doi.org/10.1017/exp.2020.67)

2020

Ferguson, K.B., Pannebakker, B.A., Centurión, A., Heuvel, J. van den, Nieuwenhuis, R., Becker, F.F.M., Schijlen, E., Thiel, A., Zwaan, B.J., Verhulst, E.C. (2020) Bracon brevicornis genome showcases the potential of linked-read sequencing in identifying a putative complementary sex determiner gene. Genes 11 (12), 1390 (DOI: 10.1101/2020.07.20.211656)
Ferguson K.B., Visser S., Dalíková M., Provazníková I., Urbaneja A., Pérez-Hedo M., Marec F., Werren J.H, Zwaan B.J., Pannebakker B.A., Verhulst E.C. (2020) Jekyll or Hyde? The genome (and more) of Nesidiocoris tenuis, a zoophytophagous predatory bug that is both a biological control agent and a pest. Insect Molecular Biology (DOI: 10.1111/imb.12688)
Zou, Y., Geuverink, E., Beukeboom, L.W., Verhulst, E.C.*, Van de Zande, L.* (2020) A chimeric gene paternally instructs female sex determination in the haplodiploid wasp Nasonia. Science 370(6520): 1115-1118 (DOI: 10.1126/science.abb8949) [Click links for: Full text | PDF]
Ferguson, K.B., Kursch-Metz, T., Verhulst, E.C., Pannebakker, B.A. (2020) Hybrid genome assembly and evidence-based annotation of the egg parasitoid and biological control agent Trichogramma brassicae. G3: Genes, Genomes, Genetics (DOI: 10.1534/g3.120.401344)
Leung, K., Ras, E., Ferguson, K.B., Ariëns, S., Babendreier, D., Bijma, P., Bourtzis, K., Brodeur, J., Bruins, M.A., Centurión, A., Chattington, S.R., Chinchilla‐Ramírez, M., Dicke, M., Fatouros, N.E., González‐Cabrera, J., Groot, T.V.M., Haye, T., Knapp, M., Koskinioti, P., Hesran, S.L., Lyrakis, M., Paspati, A., Pérez‐Hedo, M., Plouvier, W.N., Schlötterer, C., Stahl, J.M., Thiel, A., Urbaneja, A., Zande, L. van de, Verhulst, E.C., Vet, L.E.M., Visser, S., Werren, J.H., Xia, S., Zwaan, B.J., Magalhães, S., Beukeboom, L.W., Pannebakker, B.A. (2020) Next-generation biological control: the need for integrating genetics and genomics. Biological Reviews (DOI: 10.1111/brv.12641)

2019

Paspati A., Ferguson K.B., Verhulst E.C., Urbaneja A., González‐Cabrera J., Pannebakker B.A. (2019) Effect of mass rearing on the genetic diversity of the predatory mite Amblyseius swirskii. Entomologia Experimentalis et Applicata (Special Issue: Next Generation Biological Control) 167(7): 670-681 (DOI: 10.1111/eea.12811)

2018

Geuverink E., Verhulst E.C., van Leussen M., van de Zande L., Beukeboom L.W. (2018) Maternal provision of non-sex-specific transformer messenger RNA in sex determination of the wasp Asobara tabida. Insect Molecular Biology 27(1): 99-109 (DOI: 10.1111/imb.12352)

2017

Geuverink E., Rensink A.H., Rondeel I., Beukeboom L.W., van de Zande. L., Verhulst E.C. (2017) Maternal provision of transformer-2 is required for female development and embryo viability in the wasp Nasonia vitripennis. Insect Biochemistry and Molecular Biology 90: 23-33 (DOI: 10.1016/j.ibmb.2017.09.007)

2016

Verhulst E.C., Mateman A.C., Zwier M.V., Caro S.P., Verhoeven K.J.F. and van Oers K. (2016) Evidence from Pyrosequencing Indicates that Natural Variation in Animal Personality is Associated with DRD4 DNA Methylation. Molecular Ecology 25(8): 1801–1811. Data: http://dx.doi.org/10.5061/dryad.fj662

2015

Verhulst E.C. and Van de Zande L. (2015) Double nexus--Doublesex is the connecting element in sex determination. Briefings in Functional Genomics 14(6): 396-406 (DOI: 10.1093/bfgp/elv005). Invited review

2014

Verhulst E.C., and Van de Zande L. (2014) Insect Sex Determination: A Cascade of Mechanisms. Sexual Development 8(1-3): 5–6 (DOI:10.1159/000358405). Preface
Van de Zande L. and Verhulst E.C. (2014) Genomic imprinting and maternal effect genes in haplodiploid sex determination. Sexual Development 8(1-3):74-82 (DOI:10.1159/000357146).

2013

Verhulst E.C.*, Lynch J.A.*, Bopp D., Beukeboom L.W. and Van de Zande L. (2013) A new component of the Nasonia sex determining cascade is maternally silenced and regulates transformer expression. Plos One 8(5): e20497.

2012

Zwier M.V., Verhulst E.C., Zwahlen R., Beukeboom L.W. and Van de Zande L. (2012) DNA methylation plays a crucial role during early Nasonia development. Insect Molecular Biology 21(1): 129–138.
Lloyd, V.K., Brisson J.A., Fitzpatrick K.A., McEachern L.A., and Verhulst E.C. (2012) The Epigenetics of Emerging and Nonmodel Organisms. Genetics Research International 2012: 1–2 (DOI:10.1155/2012/491204). Preface

2011

Verhulst E.C., Genetic basis of sex determination in the haplodiploid wasp Nasonia vitripennis. PhD Thesis.

2010

Verhulst E.C., Van de Zande L. and Beukeboom L.W. (2010) Insect sex determination: it all evolves around transformer. Current Opinion in Genetics & Development 20(4): 376-383. Invited review
Verhulst E.C., Beukeboom L.W. and Van de Zande L. (2010) Maternal control of haplodiploid sex determination in the wasp Nasonia. Science 328(5978):620-623.
Werren J.H., Richards S., Desjardins C.A., Niehuis O., Gadau J., Colbourne J.K. and The Nasonia Genome Working Group (2010) Functional and evolutionary insights from the genomes of three parasitoid Nasonia species, Science 327(5963): 343-348.
Loehlin D.W., Oliveira D., Edwards R., Giebel J.D., Clark M.E., Cattani M.V., Van de Zande L., Verhulst E.C., Beukeboom L.W., Munoz-Torres M. and Werren J.H. (2010) Non-coding changes cause sex-specific wing size differences between closely related species of Nasonia. PLoS Genetics 6(1): e1000821 1-5.

2009

Oliveira D.C.S.G., Werren J.H., Verhulst E.C., Giebel J.D., Kamping A., Beukeboom L.W. and Van de Zande L. (2009) Identification and characterization of the doublesex gene of Nasonia. Insect Molecular Biology 18(3):315-324.

* Equal contributions

H-index: 12
ResearcherID: J-2641-2013
ORCID: 0000-0002-4781-535X

Editorials & Reviewing

Editorials

Current - Topic editor for Insects (MDPI)
Current - Reviewing editor for Experimental Results (Cambridge University Press)
2014 - Invited guest editor for a special issue of Sexual Development. Sex determination and differentiation in insects. Sexual Development 2014, Vol. 8, No. 1–3; Guest Editors: E.C. Verhulst and L. van de Zande
2012 - Invited guest editor for a special issue of Genetics Research International. The epigenetics of emerging and nonmodel organisms. Genetics Research International 2012, Article ID 491204, Guest Editors: Vett K. Lloyd, Jennifer A. Brisson, Kathleen A. Fitzpatrick, Lori A. McEachern, and Eveline C. Verhulst

Reviewer

Referee for: International Journal of Genomics; Frontiers in Zoology; Peer Community In; Molecular Phylogenetics & Evolution; Insect Science; Insects; Experimental Results; Pest Management Science; RNA Biology; BMC Genomics; Parasites & Vectors; Development, Genes and Evolution; Physiological Entomology; Plos One; Insect Molecular Biology; Proceedings of the Royal Society B.; Agence Nationale de la Recherche (ANR); Scientific Reports; Heredity; Plos Genetics; Animal Reproduction Science; Development Genes and Evolution; FEBS Letters; Nature Ecology and Evolution

Public media

Resource magazine: Riddle of the wasp solved
KIJK magazine: Gen maagdelijke geboorte honingbij ontdekt
Volkskrant: Vrouwtjesinsect ontdekt met penis en mannetjesinsect met vagina
NRC krant: Sluipwesp laat ei onbevrucht en schakelt een gen uit om zoon te krijgen